Remarkable enhancement of flavonoid production in a co-cultivation system of Isatis tinctoria L. hairy root cultures and immobilized Aspergillus niger
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چکیده
منابع مشابه
Genista tinctoria hairy root cultures for selective production of isoliquiritigenin.
Hairy root cultures were established after inoculation of Genista tinctoria in vitro shoots with Agrobacterium rhizogenes, strain ATCC 15834. In transformed roots of G. tinctoria grown in Schenk-Hildebrandt medium without growth regulators the biosynthesis of isoflavones, derivatives of genistein and daidzein, and flavones, derivatives of luteolin and apigenin, characteristic for the intact pla...
متن کاملEstablishment of Hairy Root Cultures by Agrobacterium Rhizogenes Mediated Transformation of Isatis Tinctoria L. for the Efficient Production of Flavonoids and Evaluation of Antioxidant Activities
In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR amplification of rolB, rolC and aux1 genes. Culture parameters of ITHRCs were optimized by Box-Behnken design (BBD), and eight bioactive FL constituents (r...
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Tetrahydrocannabinol (THC) derivatives are used clinically as analgesic, anti-inflammatory, appetite stimulant, anti-emetic and anti-tumor cannabinoids. THC and its related compounds are at present obtained by extraction from intact Cannabis plants or chemical synthesis, but plant cell cultures may be an alternative source of production. In the present study, hairy root cultures of C. sativa (C...
متن کاملHairy Root Cultures of Hypericum perforatum L.; A Promising Method for The High Scale Production of Hypericin
Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites. Objective: In this study, hairy roots of Hypericum Perforatum have been prepared using the seeds and bio transformed by bacteria. Fin...
متن کاملEnhancement of Tropane Alkaloid Production among Several Clones and Explants Types of Hairy Root of Atropa belladonna L.
Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...
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ژورنال
عنوان ژورنال: Industrial Crops and Products
سال: 2018
ISSN: 0926-6690
DOI: 10.1016/j.indcrop.2017.12.017